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Abstract: . . . et al. (2002, 2004a and b) in obese people indicate that the trans -10, cis -12 CLA isomer may cause significant impairment of the peripheral insulin sensitivity as well as elevation of blood glucose and serum lipid concentrations. http://www.efsa.eu.int/science/nda/nda_opinions/catindex_en.html Page 16 of 49 Page 17 Elias and Innis (2001) observed a negative correlation between the proportion of CLA in infant plasma cholesteryl esters and birth weight and length of gestation of term infants. A negative correlation . . . . . . three steps: the extraction of the fat from the complex food or tissue matrix, is followed by the conversion of the total lipids into methyl esters or other derivatives which are then analyzed by GLC or HPLC or a combination of both methods. Infrared analyses may be carried out on the lipid extract or after conversion into derivatives. Generally, most lipid analysts use a mixture of chloroform Methanol (2:1 by volume) to extract lipids from animal, plant and bacterial tissue (Folch et al. , 1957 . . . . . . consumption of CLA to be around 300 mg per day. In some countries (e.g. Australia) consumption of CLA can be up to 1.5 g/day (Parodi, 1994). There is an increasing amount of specific scientific literature on the biological effects of CLA (for current citations to the published scientific literature on CLA, see http://www.wisc.edu/fri/clarefs.htm ; see also Angel, 2004). The published animal studies and clinical trials indicate the possibility that CLA may have some health benefits, e.g. reduction of body fat gain, immune response, and improvement . . . . . . The EFSA Journal (2004) 81, 1-49 Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies on a request from the Commission related to the presence of trans fatty acids in foods and the effect on human health of the consumption of trans fatty acids (Request N° EFSA-Q-2003-022) (adopted on . . . . . . The EFSA Journal (2004) 81, 1-49 Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies on a request from the Commission related to the presence of trans fatty acids in foods and the effect on human health of the consumption of trans fatty acids (Request N° EFSA-Q-2003-022) (adopted on 8 July 2004) . . . --3000,5,300,2879,64585
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